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constrained iterative deconvolution  (Softworx Inc)

 
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    Softworx Inc constrained iterative deconvolution
    Constrained Iterative Deconvolution, supplied by Softworx Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constrained-iterative+deconvolution/deconvolution+software+softworx/bio_rxiv__64898__2026__02__09__704383-284-10-12
    Average 86 stars, based on 1 article reviews
    constrained iterative deconvolution - by Bioz Stars, 2026-09
    86/100 stars

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    other:

    Article Title: Retromer and the dynamin Vps1 cooperate in the retrieval of transmembrane proteins from vacuoles.
    Article Snippet: Z-stacks of 1-4 μm with 300 nm spacing were used for constrained-iterative deconvolution (SoftwoRx).

    Article Title: Vps39 Interacts with Tom40 to Establish One of Two Functionally Distinct Vacuole-Mitochondria Contact Sites.
    Article Snippet: Cells were washed twice with water and imaged in water.We used z-stacks with 250 nm spacing for constrained-iterative deconvolution (SoftWoRx), and the images shown are deconvolved images of one z-plane.

    Article Title: Vps39 Interacts with Tom40 to Establish One of Two Functionally Distinct Vacuole-Mitochondria Contact Sites.
    Article Snippet: We used z-stacks with 350 nm spacing for constrained-iterative deconvolution (SoftWoRx).

    Article Title: A systematic approach to identify recycling endocytic cargo depending on the GARP complex
    Article Snippet: We used constrained-iterative deconvolution (SoftWoRx).

    Article Title: Hypomyelination and developmental delay associated with VPS11 mutation in Ashkenazi-Jewish patients.
    Article Snippet: To cite: Edvardson S, Gerhard F, Jalas C, et al. J Med Genet Published Online First: [please include Day Month Year] doi:10.1136/ jmedgenet-2015-103239 ABSTRACT Background The genetic heterogeneity of developmental delay and cognitive impairment is vast.. The endocytic network is essential for neural development and synaptic plasticity by regulating the sorting of numerous transmembrane proteins.. Disruption of the pathway can lead to neuronal pathology.

    Article Title: The receptor subunit Tom20 is dynamically associated with the TOM complex in mitochondria of human cells
    Article Snippet: We used z-stacks with 250 nm spacing for constrained-iterative deconvolution (SoftWoRx).



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    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Journal: American journal of respiratory cell and molecular biology

    Article Title: Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen Challenged Mice

    doi: 10.1165/rcmb.2004-0060OC

    Figure Lengend Snippet: Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Article Snippet: Tissues were observed on an inverted microscope by focusing on 45 consecutive 0.2 μm focal planes followed by constrained-iterative deconvolution (DeltaVision, Applied Precision, LLC, Issaquah, Washington).

    Techniques: Produced, Staining, Immunolabeling, Microscopy